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Last Updated on: January 1, by Sagar Aryal. When an electric current is applied to a slide layered with gel, the antigen mixture placed in wells is separated into individual antigen components according to their charge and size.
Following electrophoresis, the separated antigens are reacted with specific antisera placed in troughs parallel to the electrophoretic migration and diffusion is allowed to occur. Antiserum present in the trough moves toward the antigen components resulting in the formation of separate precipitin lines in hrs, each indicating reaction between individual proteins with its antibody.
Table of Contents hide. Principle of Immunoelectrophoresis. Procedure of Immunoelectrophoresis. Results of Immunoelectrophoresis. Applications of Immunoelectrophoresis. Advantages of Immunoelectrophoresis. Limitations of Immunoelectrophoresis. Share the article on:. Related Notes:. Opsonization- Definition, Mechanism, Opsonins, Examples. Instruments used in Microbiology Lab with Principle and Uses. Endocytosis- Definition, Process and Types with Examples. Immune Booster Foods.
Gel Filtration Chromatography. Counter Current Immunoelectrophoresis. So good!
Immunoelectrophoresis- Principle, Procedure, Results and Applications, Advantages and Limitations
Last Updated on: January 7, by Sagar Aryal. Rocket immunoelectrophoresis is a quantitative one-dimensional single electro-immunodiffusion technique. In this method antibody is incorporated in the gel at a pH value at which the antibodies remain essentially immobile. Antigen is placed in wells cut in the gel. Electric current is then passed through the gel, which facilitates the migration of negatively charged antigens into the agar. As the antigen moves out of the well and enters the agarose gel, it combines with the antibody to form immune complex which becomes visible.
National Diagnostics continues its tradition of bringing laboratories more high quality options for scientific research by introducing three new p. National Diagnostics announces the release of a new, ultra-high sensitivity reagent for HRP mediated Western Blotting. Antibodies are produced by the immune system in response to foreign macromolecules. Each antibody binds specifically to one feature epitope on one macromolecule antigen. This allows the use of antibodies for the detection and quantitation of specific proteins in complex mixtures.
Immunoelectrophoresis is a general name for a number of biochemical methods for separation and characterization of proteins based on electrophoresis and reaction with antibodies. All variants of immunoelectrophoresis require immunoglobulins , also known as antibodies , reacting with the proteins to be separated or characterized. The methods were developed and used extensively during the second half of the 20th century. The agarose was chosen as the gel matrix because it has large pores allowing free passage and separation of proteins, but provides an anchor for the immunoprecipitates of protein and specific antibodies. The high pH was chosen because antibodies are practically immobile at high pH. An electrophoresis equipment with a horizontal cooling plate was normally recommended for the electrophoresis.