ENTEROTUBE II INTERPRETATION GUIDE PDF

It has prepared sterile multimedia tube for rapid differential identification of Gram-negative bacteria Enterobacteriaceae. The self-contained, sterile, compartmented plastic tube contains twelve different conventional media plus a self-contained inoculating wire. The checked positive numbers are totaled, and the composite number is then located in the Interpretation Guide to identify the organisms. Where two or more organisms are listed, the confirmatory tests required to further identify them are also given. Save my name and email in this browser for the next time I comment. Rapid identification of gram-negative bacilli of the family Enterobacteriaceae isolated from clinical specimens has long posed a problem for the clinical laboratory.

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Additional information for use of this Interpretation Guide is provided on the following pages. This Interpretation Guide section consists of 3 databases. The first one is a limited database for. The remaining two databases. When more than one organism is listed under a given identification result, the correct organism. Confirmatory testing is usually only necessary for identification of rare biotypes, with the. Serological typing is also recommended. Shigella, but do react with the respective typing sera should be sent to a reference laboratory for.

Instructions for these procedures and all antisera typing material are readily. Identification Database. If a rare organism is the best choice, check the purity of the isolate and repeat the.

Also, if a rare organism is encountered as the first choice,. If only one organism name appears as an identification, atypical tests and any confirmatory tests. If two or more organisms are presented without atypical test results, then confirmatory tests. If an identification result is obtained which cannot be found in the Interpretation Guide, the.

Recheck the biocode to be certain that the biochemical profile does not contain a positive result. Vorliegen eines seltenen Keimes hin. Falls alles auf einen seltenen Keim hinweist, muss die. II mit gut isolierten Kolonien. Proskauer VP compartiment 9. Seul le respect total des instructions fournies par le.

Si tout semble indiquer la. I microrganismi che sono biochimicamente coerenti. Le istruzioni per queste procedure e. Inoltre, se si riscontra un microrganismo raro come prima scelta e sono elencati anche batteri. Vengono considerati atipici tutti i. A questo. Strisciare il materiale per isolamento su un terreno non selettivo come una piastra di agar.

Inoculare quindi una. Infine controllare anche. Ricontrollare il biocodice per assicurarsi che il profilo biochimico non contenga un risultato. La primera es una. Las otras dos bases de datos se utilizan para los bacilos fermentadores de glucosa y.

Las instrucciones para dichos. Las instrucciones del fabricante deben seguirse al pie. Asimismo, si se encuentra un organismo raro. Cualquier resultado de. Esto se. Extender el material para aislamiento en un medio no selectivo, por ej. Luego, inocular un tubo. Asimismo, comprobar finalmente que el. Tal pode ser. Em seguida,.

Escherichia coli AD. Organismer, der biokemisk er konsistente med Salmonella eller Shigella,. Ethvert biokemisk testresultat fra BBL. Taip pat, jei su retu mikroorganizmu susiduriama kaip. Tai galima padaryti. Za wynik. Ak je. Enterotube II. MacFaddin, J. Biochemical Tests for identification of medical bacteria.

The Williams. Smibert, R. Phenotypic characterization. In: Gerhardt, P. Farmer II I, J. Enterobacteriaceae: introduction and identification. In: Murray, P.

Baron, J. Jorgensen, M. Pfaller, and R. Yolken ed. Manual of clinical microbiology,. Databases This Interpretation Guide section consists of 3 databases. The first one is a limited database for oxidase-negative, glucose non-fermenting Gram-negative bacilli. The remaining two databases are used for oxidase-negative, glucose fermenting bacilli Enterobacteriaceae.

Thus, the VP reaction may be considered a strictly as a confirmatory test, used only as a confirmatory test when indicated in the Identification Database method without VP , or b as an on-line test by incorporating the result of the VP test into the profile number and using the Identification Database method with VP.

Confirmatory Tests When more than one organism is listed under a given identification result, the correct organism is chosen on the basis of the results of the tests suggested in the "Confirmatory Tests" column. For abbreviations, see Table in section 4 of this chapter.

Confirmatory testing is usually only necessary for identification of rare biotypes, with the exception of confirmation testing with antisera. NOTE: If the biochemical Identification of Salmonella, Shigella, or Yersinia is obtained, the isolate should always be subjected to serological typing.

Serological typing is also recommended for Escherichia coli AD. Organisms that are biochemically consistent with Salmonella or Shigella, but do react with the respective typing sera should be sent to a reference laboratory for identification.

Instructions for these procedures and all antisera typing material are readily available commercially, and manufacturers' instructions should be followed closely to ensure proper results. Rare Organisms The organisms identification corresponding to the 5-digit profile number is listed in the Identification Database.

If a rare organism is the best choice, check the purity of the isolate and repeat the inoculation of the BBL Enterotube II. Also, if a rare organism is encountered as the first choice, and common microbes are also listed, confirmatory tests must be done. The frequency of occurrence of these rare isolates is so low often less than once per year that a technical error such as a mixed or light inoculum should be ruled out before reporting the results.

If only one organism name appears as an identification, atypical tests and any confirmatory tests will be listed, but a confidence value will not appear since this is considered a satisfactory identification.

If two or more organisms are presented without atypical test results, then confirmatory tests must be performed to obtain an identification. If an identification result is obtained which cannot be found in the Interpretation Guide, the following should be considered: Recheck that the organism used for the inoculation is a pure culture.

This can be done by carefully melting the adhesive cover film with a hot wire or loop and collecting some growth of the culture from the citrate or urea compartment.

Streak the material for isolation on a nonselective medium, e. Also, eventually check that the organism is a Gram negative rod. Recheck the biocode to be certain that the biochemical profile does not contain a positive result for both phenylalanine deaminase and dulcitol.

They are generally nonmotile, lactose-negative and do not form gas. Identifications of Shigella species must always be confirmed with serological testing. Identifications of Salmonella species must always be confirmed with serological testing. Datenbanken Dieser Teil des Codebuches besteht aus drei Datenbanken.

Beachten Sie: Wenn Salmonella, Shigella, oder Yersinia biochemisch identifiziert wurden, sollte der isolierte Keim immer noch einer serologischen Typisierung unterzogen werden.

Streichen Sie dieses Material zur Isolierung auf einem nichtselektiven Medium, z. Sie sind normalerweise unbeweglich, Lactose-negativ und bilden kein Gas.

Per le abbreviazioni, vedere la tabella nella sezione 4 di questo capitolo. Inoltre, se si riscontra un microrganismo raro come prima scelta e sono elencati anche batteri comuni, occorre eseguire i test di conferma. Vengono considerati atipici tutti i risultati dei test biochimici ottenuti dal sistema BBL Enterotube II e ritenuti improbabili per una data specie quando comparati con la tabella delle reazioni.

A questo scopo, usare un'ansa o un filo riscaldato per sciogliere con attenzione la pellicola di rivestimento adesivo e prelevare un'aliquota di crescita della coltura dallo scomparto del citrato o dell'urea. Strisciare il materiale per isolamento su un terreno non selettivo come una piastra di agar sangue , incubare per 18 - 48 ore e verificare la purezza della coltura.

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